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产品描述：人免疫球蛋白G4(IgG4)酶联免疫试剂盒，为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgG4含量。IgG4是一种免疫球蛋白，在免疫调节中发挥独特作用。其背景上，IgG4异常与多种疾病相关。研究机制方面，它亲和力成熟高，能发生Fab臂交换形成不对称抗体，可阻断抗原抗体相互作用，目前在自身免疫病、肿瘤等研究中受关注。试剂盒检测范围为39 ng/mL-10000 ng/mL，该产品适用于科研领域中对IgG4表达水平的体外研究，例如探索其在免疫调节、过敏反应或纤维化疾病中的生物学作用，也可作为评估实验模型中免疫应答特征的辅助工具，为炎症机制研究、生物标志物筛选及药物开发提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgG4
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：39 ng/mL-10000 ng/mL
灵敏度：39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IgG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:2000 | Average % | 99 | ||||
| Range % | 93-104 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8000 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human IgG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 100000.2140.2160.215 25000.5980.6010.600 6250.9981.0131.006 156.251.5971.6141.606 391.8691.8891.879 02.3442.3682.356 | ||||||
货期：3-5 working days
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