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产品描述：猪免疫球蛋白M(IgM)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgM含量。IgM是人体最早产生的免疫球蛋白，在抗感染早期发挥重要作用，可高效激活补体系统。其作为靶点的研究机制主要是基于它与疾病的关联，通过设计药物或抗体与之结合，调节免疫反应，为自身免疫病、感染性疾病等的治疗打开新思路。试剂盒检测范围为0.039 μg/mL-10 μg/mL，常用于科研领域评估疫苗效果、感染性疾病模型构建或免疫机制研究；可为猪类免疫相关研究提供可靠数据支持，例如传染病防控、免疫增强剂开发或营养干预实验中的免疫指标监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgM
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.039 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of pig IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 104 | 98-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 100.2040.2060.205 2.50.4630.4540.459 0.620.9150.9360.926 0.161.4291.4881.459 0.042.0562.1342.095 02.5152.6522.584 | ||||||
货期：3-5 working days
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