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产品描述：人印度刺猬因子(IHH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IHH含量。试剂盒检测范围为1.56 pg/mL-100 pg/mL，支持科研领域对IHH表达调控机制的研究，例如骨骼发育模型构建、肿瘤微环境分析或代谢性疾病相关分子机制探索，适用于基础医学、分子生物学等领域的体外实验研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BDA1 ELISA Kit; HHG-2 ELISA Kit; HHG2 ELISA Kit; IHH ELISA Kit; IHH_HUMAN ELISA Kit; Indian Hedgehog ELISA Kit; Indian hedgehog homolog ELISA Kit; Indian hedgehog protein ELISA Kit; Indian hedgehog protein C-product ELISA Kit; Indian hedgehog protein N-product ELISA Kit
缩写：IHH
Uniprot No.：Q14623
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 96-107 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 90-95 | |||||
回收率：
| The recovery of human IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-103 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.5602.5552.5582.442 502.0131.9691.9911.875 251.2751.2651.2701.154 12.50.5970.5830.5900.474 6.250.3790.3700.3750.259 3.120.2410.2340.2380.122 1.560.1860.1780.1820.066 00.1180.1140.116 | ||||||
货期：3-5 working days
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