首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素12(IL-12/P40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12B含量。IL12B是重要靶点。它参与免疫调节，所编码的蛋白是IL-12和IL-23等细胞因子的亚基。研究机制上，围绕其在自身免疫疾病、肿瘤免疫等领域展开，通过调控相关细胞因子活性，有望为相关疾病治疗提供新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于基础科研场景，如小鼠感染模型、炎症性疾病造模后的组织微环境分析，或药物干预实验中细胞因子网络调控研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il12bInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写：IL12B
Uniprot No.：P43432
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:10 | Average % | 90 | ||||
| Range % | 85-92 | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7012.8522.7772.6335002.0052.0762.0411.8972501.2481.3161.2821.1381250.7540.7040.7290.58562.50.4760.4500.4630.31931.20.3110.3230.3170.17315.60.2520.2590.2560.11200.1480.1390.144 | ||||||
货期：3-5 working days
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