Mouse Interleukin 17,IL-17 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04608m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素17(IL-17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17A含量。IL17A是重要的促炎细胞因子。其背景在于它参与多种自身免疫疾病的发病进程。研究机制方面，IL17A可刺激上皮细胞、内皮细胞、成纤维细胞等分泌促炎因子和趋化因子，引发炎症反应，目前也是研发治疗相关疾病药物的热门靶点。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。适用于炎症机制研究、免疫调节药物开发或疾病动物模型评估等科研场景，为探索 Th17 细胞介导的免疫反应提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il17a ELISA Kit; Ctla8 ELISA Kit; Il17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit
缩写：
IL17A
Uniprot No.：
Q62386
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	90
	Range %	85-100
1:4	Average %	96
	Range %	92-99
1:8	Average %	97
	Range %	92-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.702	2.604	2.653	2.494
2500	2.055	1.967	2.011	1.852
1250	1.386	1.418	1.402	1.243
625	0.875	0.829	0.852	0.694
312	0.537	0.521	0.529	0.370
156	0.351	0.353	0.352	0.193
78	0.246	0.234	0.240	0.081
0	0.162	0.155	0.159