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产品描述：牛白介素18(IL18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL18含量。IL-18是一种促炎细胞因子，在先天性和适应性免疫反应中发挥关键作用。它可诱导Th1细胞产生IFN-γ，参与免疫调节。多个研究表明，IL-18在多种炎症性疾病、自身免疫病和肿瘤的发病机制中起重要作用，是极具潜力的治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于研究牛感染性疾病模型（如病毒或细菌感染）、免疫调节机制探索、疫苗效力评估等科研场景，也可用于评估营养干预或环境应激对牛免疫状态的影响。通过标准化操作流程，研究者可快速获得IL18在免疫相关研究中的动态变化数据，为深入解析牛类免疫系统功能提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：IL18
Uniprot No.：Q9TU73
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 83-92 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 92-99 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 87-98 | ||||||
回收率：
| The recovery of bovine IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 90-110 | |||||
| EDTA plasma (n=4) | 97 | 90-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20003.0112.9782.9952.90010002.5112.5222.5172.4225001.4961.5041.5001.4052500.7050.7160.7110.6161250.3820.3750.3790.28462.50.2260.2340.2300.13531.250.1520.1590.1560.06100.0930.0970.095 | |||||||
货期：3-5 working days
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