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产品描述：猪白介素18(IL-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL18含量。IL18 即白细胞介素 18，是一种多功能促炎细胞因子，在先天免疫和获得性免疫中均发挥重要作用。其研究机制主要围绕它能诱导 T 细胞和自然杀伤细胞产生γ-干扰素，增强自然杀伤细胞的细胞毒活性，在炎症、免疫相关疾病研究中备受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景中动态监测实验动物模型（如细菌/病毒感染、免疫调节研究）的免疫应答水平；为兽医学、比较免疫学及动物疾病模型研究提供可靠工具，助力科研人员解析IL - 18在猪免疫系统中的分子机制与调控网络。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：IL18
Uniprot No.：O19073
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 94 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5622.6872.6252.44310001.8391.8651.8521.6705001.1041.1351.1200.9382500.6430.6380.6410.4591250.4120.4350.4240.24262.50.3210.3410.3310.14931.250.2620.2480.2550.07300.1810.1830.182 | ||||||
货期：3-5 working days
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