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产品描述：绵羊白介素1β(IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B，即白细胞介素-1β，是一种重要的促炎细胞因子，参与先天免疫反应。它由免疫和非免疫细胞在炎症信号刺激下产生，通过炎症小体激活和蛋白水解加工，与多种细胞因子协同作用，调节炎症和免疫反应，对肿瘤的发生、发展和转移具有重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可借助定量分析感染模型、自身免疫疾病研究或药物干预实验中不同生物样本的IL - 1β浓度变化，为炎症机制探索、免疫调控研究及生物制剂开发提供可靠数据支持。所有检测结果仅用于科学研究目的。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：IL1B
Uniprot No.：P21621
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-90 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of sheep IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 97 | 94-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4872.4562.4722.3715002.0312.0002.0161.9152501.6141.5681.5911.4901251.1051.0231.0640.96362.50.7030.6890.6960.59531.20.4210.4020.4120.31115.60.2750.2620.2690.16800.1030.0990.101 | ||||||
货期：3-5 working days
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