首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：鸡白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-2含量。IL-2是重要的免疫调节细胞因子，在免疫系统中作用关键。其背景上，它能参与T细胞、B细胞等免疫细胞的增殖分化。研究机制方面，主要通过与细胞表面IL-2受体结合，激活下游信号通路，从而调节免疫细胞功能，在肿瘤免疫、自身免疫病治疗研究中受关注。试剂盒检测范围为0.18 pg/mL-12 pg/mL，科研人员可将其应用于家禽免疫机制研究、疫苗效力评估或疾病模型中细胞免疫状态的动态监测，特别适用于分析感染性疾病、炎症反应及免疫调节实验中IL - 2的表达水平变化。为实验室开展禽类免疫学研究提供了标准化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-2
Uniprot No.：O73883
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.18 pg/mL-12 pg/mL
灵敏度：0.04 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 92-109 | ||||
| EDTA plasma (n=4) | 96 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 122.1512.1982.1752.072 61.8351.8691.8521.749 31.4571.4871.4721.369 1.51.0981.0511.0750.972 0.750.6980.6820.6900.587 0.380.4490.4600.4550.352 0.180.2360.2220.2290.126 00.1020.1040.103 | ||||||
货期：3-5 working days
×
