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产品描述：人白介素-21(IL-21)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL21含量。IL-21是一种细胞因子，在免疫系统中扮演着重要角色。它可调节多种免疫细胞的功能，如T细胞、B细胞和NK细胞等。相关研究围绕其信号通路展开，探究其在免疫调节、肿瘤免疫以及自身免疫病发生发展中的机制和潜在应用。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研场景中IL-21的体外定量分析，例如免疫机制研究、炎症或感染性疾病模型中细胞因子动态变化监测，以及药物或生物制剂干预后的免疫应答评估，为免疫学、分子生物学等基础研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CVID11 ELISA Kit; IL 21 ELISA Kit; IL-21 ELISA Kit; Il21 ELISA Kit; IL21_HUMAN ELISA Kit; Interleukin 21 ELISA Kit; Interleukin-21 ELISA Kit; interleukin-21 isoform ELISA Kit; Interleukin21 ELISA Kit; OTTHUMP00000164088 ELISA Kit; Za11 ELISA Kit
缩写：IL21
Uniprot No.：Q9HBE4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-100 | |||||
回收率：
| The recovery of human IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 101 | 85-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4452.3452.3952.2501001.8641.7641.8141.669501.1621.1511.1571.012250.7230.7130.7180.57312.50.4320.4220.4270.2826.250.3150.3050.3100.1653.120.2220.2120.2170.07200.1450.1440.145 | ||||||
货期：3-5 working days
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