首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素23(IL-23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-23含量。IL-23是重要治疗靶点。它是一种促炎细胞因子，在自身免疫病发病机制中起关键作用。研究发现阻断IL-23信号通路可调节免疫反应，有望为银屑病、炎症性肠病等疾病治疗带来新方法，目前相关药物研发是热点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，科研人员可将其用于免疫机制研究、炎症性疾病模型分析、药物靶点筛选等基础研究领域，特别适用于评估实验动物或细胞模型中IL - 23的表达变化及其在病理生理过程中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IL-23
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-96 | ||||
| EDTA plasma (n=4) | 93 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.6082.5072.5582.4152001.8651.7641.8151.6721001.2071.1861.1971.054500.8010.7900.7960.653250.4370.4480.4430.30012.50.3010.3120.3070.1646.250.2280.2340.2310.08800.1420.1430.143 | ||||||
货期：3-5 working days
×
