首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素23(IL-23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-23含量。IL-23是一种细胞因子，在免疫系统中发挥重要作用。它能够调节Th17细胞的生成与功能，与自身免疫疾病、炎症反应密切相关。目前针对IL-23的研究聚焦于其信号通路及作用机制，以开发相关治疗药物来改善炎症和免疫异常状况。试剂盒检测范围为7.8 pg/ml-500 pg/ml，适用于科研领域中对感染性疾病模型、炎症反应机制、免疫调节研究的IL - 23动态监测，也可用于评估药物或生物制剂对IL - 23通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IL-23
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/ml-500 pg/ml
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 81-92 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 84-97 | ||||||
| 1:8 | Average % | 106 | |||||
| Range % | 97-110 | ||||||
回收率：
| The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 94-108 | |||||
| EDTA plasma (n=4) | 101 | 94-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 5002.8682.6892.7792.654 2501.5191.5861.5531.428 1250.7460.7000.7230.598 62.50.4340.4560.4450.320 31.20.3140.3010.3080.183 15.60.2070.2020.2050.080 7.80.1530.1590.1560.031 00.1270.1230.125 | |||||||
货期：3-5 working days
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