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产品描述：小鼠白介素3(IL-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL3含量。IL3即白细胞介素-3，是一种造血生长因子。其背景在于它能调控造血干细胞和祖细胞的增殖、分化与存活。研究机制方面，IL3与细胞表面特异性受体结合，激活下游信号通路，进而影响多种血细胞的生成和功能，在血液学研究领域受广泛关注。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。适用于科研领域中对免疫调控机制、炎症性疾病模型或药物干预效果的评估，例如通过检测小鼠感染模型、过敏反应或造血系统研究中IL-3的动态变化，为体外细胞培养上清、动物实验血清或组织样本提供高特异性检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il3 ELISA Kit; Csfmu ELISA Kit; Il-3 ELISA Kit; Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
缩写：IL3
Uniprot No.：P01586
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 83-92 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 91-100 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 86-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of mouse IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-98 | |||||
| EDTA plasma (n=4) | 97 | 86-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.6952.6802.6882.5112501.8851.8671.8761.6991251.3021.2951.2991.12262.50.7580.7440.7510.57431.20.4990.4920.4960.31915.60.3560.3590.3580.1817.80.2810.2870.2840.10700.1750.1790.177 | |||||||
货期：3-5 working days
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