| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPAG7 |
| Uniprot No | O75391 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADLLGS ILSSMEKPPS LGDQETRRKA REQAARLKKL QEQEKQQKVE FRKRMEKEVS DFIQDSGQIK KKFQPMNKIE RSILHDVVEV AGLTSFSFGE DDDCRYVMIF KKEFAPSDEE LDSYRRGEEW DPQKAEEKRK LKELAQRQEE EAAQQGPVVV SPASDYKDKY SHLIGKGAAK DAAHMLQANK TYGCVPVANK RDTRSIEEAM NEIRAKKRLR QSGEELPPTS |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPAG7重组蛋白的示例参考文献(注：部分信息为假设性概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *"Molecular characterization and recombinant expression of SPAG7: Implications for sperm function"*
**作者**: Saxena, D., et al.
**摘要**: 本研究克隆了人类SPAG7基因，并在大肠杆菌中成功表达重组SPAG7蛋白。通过体外功能实验发现，该蛋白在精子运动性和顶体反应中起关键作用，为男性不育症研究提供了新靶点。
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2. **文献名称**: *"SPAG7 interacts with HSP70 in cervical cancer cells: Role of recombinant SPAG7 in apoptosis regulation"*
**作者**: Chaurasiya, S.K., et al.
**摘要**: 利用昆虫细胞系统表达纯化的重组SPAG7蛋白，揭示了其与热休克蛋白HSP70的相互作用。实验表明，SPAG7过表达可诱导宫颈癌细胞凋亡，提示其潜在的肿瘤抑制功能。
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3. **文献名称**: *"Structural and functional analysis of SPAG7 using recombinant protein technology"*
**作者**: Kumar, R., et al.
**摘要**: 通过原核表达系统获得高纯度SPAG7重组蛋白，结合X射线晶体学解析其三维结构。功能研究表明，SPAG7的特定结构域参与DNA结合，可能影响染色质重塑过程。
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4. **文献名称**: *"Development of SPAG7-specific monoclonal antibodies using recombinant antigen"*
**作者**: Li, Y., et al.
**摘要**: 以重组SPAG7蛋白免疫小鼠，成功制备多克隆及单克隆抗体。抗体验证实验显示高特异性，为SPAG7在生殖组织和肿瘤中的定位研究提供了可靠工具。
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**建议**：上述内容为示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“SPAG7 recombinant protein”或“SPAG7 expression”)获取最新研究。
SPAG7 (sperm-associated antigen 7) is a protein primarily associated with spermatogenesis and male reproductive biology. Initially identified in testicular tissues, it plays a critical role in sperm development, particularly in the structural organization of the sperm head and the acrosome, a vesicle essential for fertilization. SPAG7 is localized to the sperm's fibrous sheath and nucleus, suggesting involvement in sperm motility and chromatin stability. Dysregulation of SPAG7 has been linked to male infertility, making it a potential biomarker for diagnosing sperm quality issues.
Recombinant SPAG7 protein is engineered using biotechnological methods, typically expressed in bacterial or mammalian systems (e.g., E. coli or HEK293 cells) to ensure proper folding and post-translational modifications. The recombinant form retains functional epitopes, enabling its use in antibody production, diagnostic assays, and functional studies. Researchers employ it to investigate SPAG7's interactions with other proteins (e.g., AKAPs in signaling pathways) and its role in cellular processes like meiosis or DNA repair.
Recent studies explore SPAG7's broader implications beyond reproduction. Its expression in certain cancers (e.g., ovarian, cervical) hints at oncogenic roles, though mechanisms remain unclear. Structural analyses of recombinant SPAG7 aim to map functional domains, aiding drug design for infertility or cancer therapies. As a research tool, it bridges gaps in understanding gametogenesis and cellular dynamics, highlighting its dual significance in basic and clinical sciences.
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