Mouse Interleukin 33(IL-33) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E14393m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素33(IL-33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL33含量。IL33即白细胞介素 33，是IL-1家族成员。它作为一种警报素，在组织受损或应激时释放。其研究机制主要是通过与ST2受体结合，激活NF-κB和MAPK信号通路，参与Th2免疫反应，在哮喘、过敏性疾病等多种炎症疾病中发挥重要作用。试剂盒检测范围为15.6 pg/ml- 1000 pg/ml，灵敏度为3.9 pg/ml。适用于基础医学研究中IL-33在哮喘模型、特应性皮炎动物实验、肠道炎症病理机制探索等领域的表达动力学研究，也可用于评估药物干预或基因修饰对IL-33信号通路的影响，为免疫调控相关科研提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il33Interleukin-33 ELISA Kit; IL-33) [Cleaved into: Interleukin-33(102-266); Interleukin-33(109-266)] ELISA Kit
缩写：
IL33
Uniprot No.：
Q8BVZ5
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/ml - 1000 pg/ml
灵敏度：
3.9 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	100
	Range %	93-105
1:2	Average %	97
	Range %	92-103
1:4	Average %	104
	Range %	97-111
1:8	Average %	100
	Range %	93-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.963	2.834	2.899	2.721
500	2.218	2.167	2.192	2.015
250	1.700	1.658	1.679	1.502
125	1.184	1.159	1.172	0.994
62.5	0.648	0.671	0.659	0.482
31.2	0.404	0.413	0.408	0.231
15.6	0.289	0.284	0.286	0.109
0	0.175	0.180	0.177