首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素-34(IL34)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的IL34含量。IL34作为靶点，它是一种细胞因子。其背景在于它在免疫调节中发挥重要作用。研究机制方面，它能与特定受体结合，激活相关信号通路，参与调控免疫细胞的生长、分化和功能，在炎症、肿瘤等多种疾病发生发展过程中可能扮演关键角色。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于科研领域对IL - 34表达水平的动态分析；支持体外细胞模型研究（如免疫细胞激活实验）、动物疾病模型（如炎症或肿瘤模型）的组织样本检测，以及临床前研究中体液生物标志物的定量评估，为探索IL - 34在免疫调控、疾病机制及药物开发中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C16orf77 ELISA Kit; Chromosome 16 open reading frame 77 ELISA Kit; FPT025 ELISA Kit; IL 34 ELISA Kit; IL-34 ELISA Kit; Il34 ELISA Kit; IL34_HUMAN ELISA Kit; Interleukin 34 ELISA Kit; Interleukin-34 ELISA Kit; Interleukin34 ELISA Kit; MGC34647 ELISA Kit
缩写：IL34
Uniprot No.：Q6ZMJ4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:4 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 15002.7562.8202.7882.696 7502.2482.1462.1972.105 3751.5371.6011.5691.477 187.50.9120.9550.9340.842 93.750.4910.4680.4800.388 46.880.2740.2820.2780.186 23.440.1850.1790.1820.090 00.0890.0940.092 | |||||||
货期：3-5 working days
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