首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素-34(IL34)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL34含量。IL34是一个重要靶点。它是一种细胞因子，能与受体结合激活相关信号通路。研究发现其在免疫调节、炎症反应等过程中有重要作用，还和多种疾病的发生发展相关。对IL34作用机制的研究，有助于开发新的疾病治疗策略与药物。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研中探索炎症模型、自身免疫性疾病机制或药物干预效果评估，支持科研人员通过多类型生物样本（如血液或组织提取物）开展系统性研究，为深入解析IL - 34在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il34Interleukin-34 ELISA Kit; IL-34 ELISA Kit
缩写：IL34
Uniprot No.：Q4KM46
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 107 | 103-110 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.1002.1682.1341.9958001.6541.6041.6291.4904001.1811.2041.1931.0542000.7650.7780.7720.6331000.5170.5020.5100.371500.3460.3510.3490.210250.2280.2310.2300.09100.1400.1380.139 | |||||||
货期：3-5 working days
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