首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素35(IL-35)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL-35含量。IL-35是IL-12家族的新成员，由EBI3和p35亚基组成。它在免疫调节中发挥关键作用，与IL12B形成IL-12细胞因子或与EBI3/IL27B形成IL-35细胞因子。IL-35在多种炎症和肿瘤中表达异常，其作用机制涉及调节T细胞和自然杀伤细胞反应，以及诱导干扰素γ的产生。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞因子分泌分析、实验动物模型血清/组织IL-35动态监测等场景；为研究IL-35在免疫调控机制、疾病模型中的功能解析及药物干预效果评价提供了高效工具，广泛应用于免疫学、肿瘤学及炎症相关基础研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-35
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-98 | ||||
| EDTA plasma (n=4) | 96 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4612.3912.4262.2355002.0731.9652.0191.8282501.6691.5771.6231.4321251.2721.1831.2281.03762.50.8140.8040.8090.61831.20.5670.5250.5460.35515.60.3350.3140.3250.13400.1940.1880.191 | ||||||
货期：3-5 working days
×
