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产品描述：牛白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL4是一个重要靶点。它是白细胞介素家族成员，在免疫系统中起关键作用，参与调节B细胞、T细胞等免疫细胞的生长和分化。研究机制上，主要聚焦其信号通路及与疾病发生发展关联，围绕它开发的药物有望用于哮喘、特应性皮炎等免疫相关疾病治疗。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域探究牛类感染性疾病、过敏反应或炎症过程中IL-4的动态变化，例如通过血清/血浆样本评估疫苗免疫效果，或利用组织匀浆分析特定器官的免疫应答机制，为畜牧养殖中的疾病机制研究和免疫调控提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：IL4
Uniprot No.：P30367
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 89 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8001.9931.9121.9531.8354001.5761.6161.5961.4782001.3041.3541.3291.2111000.9390.9620.9510.833500.6240.6410.6330.515250.4410.4190.4300.31212.50.2830.2790.2810.16300.1190.1170.118 | ||||||
货期：3-5 working days
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