Mouse Interleukin 4,IL-4 ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04634m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL4是重要的细胞因子，在免疫调节等方面有重要作用。它可调控Th2细胞分化，参与过敏反应等。研究机制上围绕其信号通路展开，明确其与受体结合及下游信号激活过程，有望为免疫相关疾病如哮喘、特应性皮炎等治疗提供靶点。试剂盒检测范围为31.25 pg/ml-2000 pg/ml.，灵敏度为7.8 pg/ml。本试剂盒适用于基础免疫学研究、疾病动物模型构建（如哮喘、特应性皮炎等Th2型疾病）、药物干预效果评估等科研场景，为探究IL - 4在免疫应答中的调控机制及炎症相关疾病研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il4 ELISA kit; Il-4Interleukin-4 ELISA kit; IL-4 ELISA kit; B-cell IgG differentiation factor ELISA kit; B-cell growth factor 1 ELISA kit; B-cell stimulatory factor 1 ELISA kit; BSF-1 ELISA kit; IGG1 induction factor ELISA kit; Lymphocyte stimulatory factor 1 ELISA kit
缩写：
IL4
Uniprot No.：
P07750
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml-2000 pg/ml.
灵敏度：
7.8 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-97
1:2	Average %	97
	Range %	90-105
1:4	Average %	94
	Range %	92-96
1:8	Average %	90
	Range %	86-95

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.615	2.634	2.625	2.463
1000	1.764	1.697	1.730	1.568
500	1.311	1.228	1.270	1.107
250	0.824	0.829	0.826	0.664
125	0.581	0.621	0.601	0.439
62.5	0.313	0.303	0.308	0.146
31.25	0.257	0.244	0.250	0.088
0	0.159	0.165	0.162