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产品描述：猪白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL4含量。IL4是重要的靶点。它是白细胞介素家族成员，参与免疫调节、炎症反应等多种生理病理过程。研究机制上，IL4与其受体结合后，激活细胞内信号通路，影响Th2细胞分化等。对其研究有助于理解相关疾病发病机制及开发治疗药物。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品可广泛应用于猪免疫相关疾病模型构建、疫苗效力评估、炎症机制研究等科研领域，为探索IL - 4在感染性疾病、自身免疫性疾病及过敏性反应中的作用提供可靠工具，尤其适用于比较医学研究和畜牧兽医领域的免疫学实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：IL4
Uniprot No.：Q04745
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 92 | 86-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.7192.6522.6862.4922001.9621.8611.9121.7181001.2131.2041.2091.015500.7480.6890.7190.525250.5510.5270.5390.34512.50.3550.3740.3650.1716.250.2950.2720.2840.09000.1920.1960.194 | ||||||
货期：3-5 working days
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