Rat Interleukin 4,IL-4 ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04635r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL-4是一种由CD4+T细胞产生的细胞因子，主要参与Th2型免疫反应，诱导B细胞发育和M2型巨噬细胞分化。其研究机制涉及IL-4与IL-4R结合，激活JAK激酶和STAT6，进而调节多种免疫相关基因的表达。IL-4在过敏性疾病、肿瘤和免疫调节中发挥重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于科研领域中对炎症性疾病模型、免疫调控机制或药物干预效果的评估；为研究者探索免疫相关疾病的分子机制、疫苗开发中的 Th2 型免疫应答分析，以及环境因素对免疫系统影响的实验研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il4 ELISA Kit; Il-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell IGG differentiation factor ELISA Kit; B-cell growth factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：
IL4
Uniprot No.：
P20096
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-110
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	94-104
1:8	Average %	93
	Range %	89-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	3.065	2.834	2.949	2.794
500	2.708	2.567	2.637	2.482
250	2.135	2.028	2.082	1.926
125	1.434	1.429	1.431	1.276
62.5	0.774	0.721	0.748	0.592
31.2	0.468	0.453	0.461	0.305
15.6	0.307	0.294	0.300	0.145
0	0.156	0.155	0.155