首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素5(IL-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL5含量。IL-5 是一种重要的细胞因子，在嗜酸性粒细胞的生长、分化及存活中发挥关键作用。其过度表达与多种炎症性疾病如哮喘、嗜酸性粒细胞增多症等相关。研究聚焦于阻断 IL-5 信号通路，以减少嗜酸性粒细胞数量，从而缓解相关疾病的炎症症状。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研领域中IL-5相关免疫调节机制研究，如构建哮喘动物模型时评估炎症因子水平，或体外药物筛选实验中监测细胞因子分泌动态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写：IL5
Uniprot No.：Q08125
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 83-99 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 93-107 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-104 | |||||
回收率：
| The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-94 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4072.4572.4322.2685001.7681.7361.7521.5882501.0651.0981.0820.9181250.7040.7350.7200.55662.50.4480.4690.4590.29531.20.3230.3000.3120.14815.60.2210.2290.2250.06100.1610.1670.164 | ||||||
货期：3-5 working days
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