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产品描述：犬白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL6是重要的细胞因子，在免疫调节、炎症反应等生理病理过程中起关键作用。研究发现其异常激活与多种疾病如自身免疫病、肿瘤等密切相关。其作用机制主要是通过与受体结合激活下游信号通路，影响细胞增殖、分化和免疫功能。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于动物免疫学研究、炎症模型建立、疫苗效力评估及生物制剂开发等科研领域，为探索犬类疾病机制或药物干预效果提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：P41323
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-105 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of dog IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-96 | ||||
| EDTA plasma (n=4) | 93 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.9542.9892.9722.855502.3882.3862.3872.270251.7581.7961.7771.66012.51.0351.1061.0710.9546.250.5020.5130.5080.3913.120.2710.2680.2700.1531.560.1840.1960.1900.07300.1120.1210.117 | ||||||
货期：3-5 working days
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