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产品描述：猪白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL6是一种多功能细胞因子，在免疫、组织再生和新陈代谢中发挥重要作用。它通过经典信号转导和反式信号传导影响多种细胞功能，参与炎症、免疫反应、急性期反应和代谢调节。IL6在多种疾病中起关键作用，如炎症性疾病、自身免疫性疾病和癌症，因此成为药物研发的重要靶点。试剂盒检测范围为1.25 pg/mL-80 pg/mL，科研人员可将其应用于猪类疾病模型研究，如评估细菌或病毒感染后的免疫应答动态，分析炎症性疾病机制，或探究营养干预、疫苗效果等实验条件下IL - 6的表达变化，为动物免疫学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：P26893
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.31 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 95-103 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 99 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 802.2122.1012.1572.066 401.7251.7041.7151.624 201.2231.1941.2091.118 100.8710.8090.8400.749 50.5190.5040.5120.421 2.50.3320.3150.3240.233 1.250.1920.1820.1870.096 00.0930.0890.091 | ||||||
货期：3-5 working days
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