Mouse Interleukin-7,IL-7 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E10257m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素7(IL-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL7含量。IL-7即白细胞介素-7，是一种在免疫系统中发挥关键作用的细胞因子。它可促进淋巴细胞的存活、增殖和分化，尤其对T细胞和B细胞发育至关重要。研究聚焦其信号通路机制及在免疫治疗中的应用，以期用于肿瘤、自身免疫病等治疗。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，灵敏度为0.039 ng/ml。常用于免疫学基础研究与疾病机制探索；适用于评估实验动物模型（如免疫缺陷、自身免疫性疾病或感染模型）中IL - 7的动态变化；为研究淋巴细胞增殖、免疫微环境调控或药物干预效果提供可靠工具；广泛应用于基础医学、免疫学及生物治疗等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il7 ELISA Kit; Il-7 ELISA Kit; Interleukin-7 ELISA Kit; IL-7 ELISA Kit
缩写：
IL7
Uniprot No.：
P10168
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.156 ng/ml - 10 ng/ml
灵敏度：
0.039 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	105
	Range %	100-110
1:2	Average %	88
	Range %	84-92
1:4	Average %	107
	Range %	100-113
1:8	Average %	83
	Range %	80-85

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.681	2.600	2.641	2.511
5	2.042	2.102	2.072	1.942
2.5	1.448	1.400	1.424	1.294
1.25	0.885	0.901	0.893	0.763
0.625	0.552	0.562	0.557	0.427
0.312	0.333	0.341	0.337	0.207
0.156	0.274	0.268	0.271	0.141
0	0.131	0.129	0.130