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产品描述：牛白介素8(IL-8)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL8含量。CXCL8 是一种趋化因子，在炎症反应和免疫调节中起关键作用。它能吸引中性粒细胞等免疫细胞到炎症或损伤部位。在研究机制方面，主要聚焦其与受体结合激活下游信号通路，影响细胞迁移、增殖等，在肿瘤、炎症疾病治疗上有潜在研究价值。试剂盒检测范围为50 pg/mL-2000 pg/mL，适用于兽医学研究、畜牧业动物健康评估及疫苗开发等科研领域，为探索牛类炎症相关疾病的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写：CXCL8
Uniprot No.：P79255
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：50 pg/mL-2000 pg/mL
灵敏度：50 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 86-100 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 80-94 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of bovine IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-106 | ||||
| EDTA plasma (n=4) | 98 | 94-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.6552.5142.585 501.2791.2321.256 1500.6110.6380.625 4600.3420.3630.353 10000.2180.2240.221 20000.1250.1310.128 | ||||||
货期：3-5 working days
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