| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSSCA1 |
| Uniprot No | O60232 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMALNGAEVDDFSWEPPTEAETKVLQAR RERQDRISRLMGDYLLRGYRMLGETCADCGTILLQDKQRKIYCVACQELD SDVDKDNPALNAQAALSQAREHQLASASELPLGSRPAPQPPVPRPEHCEG AAAGLKAAQGPPAPAVPPNTDVMACTQTALLQKLTWASAELGSSTSLETS IQLCGLIRACAEALRSLQQLQH |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SSSCA1重组蛋白的3篇参考文献示例(注：文献信息为虚构示例，仅供参考)：
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1. **文献名称**: "Cloning and Expression of Recombinant SSSCA1 Protein in Escherichia coli"
**作者**: Zhang L, Wang Y, Chen H
**摘要**: 本研究成功克隆了SSSCA1基因，并在大肠杆菌中实现了高效表达。通过亲和层析纯化获得高纯度重组蛋白，验证了其抗原性，为后续抗体开发及功能研究提供了基础材料。
2. **文献名称**: "Functional Characterization of SSSCA1 in Tumor Cell Proliferation"
**作者**: Lee S, Kim T, Park J
**摘要**: 文章发现重组SSSCA1蛋白通过调控细胞周期相关信号通路促进肿瘤细胞增殖。通过体外实验证实，抑制SSSCA1表达可显著降低癌细胞生长速率，提示其作为潜在肿瘤治疗靶点的可能性。
3. **文献名称**: "Structural Analysis of SSSCA1 by X-ray Crystallography"
**作者**: Smith R, Johnson A, Brown K
**摘要**: 本研究解析了重组SSSCA1蛋白的晶体结构，揭示了其独特的α-螺旋结构域和潜在的蛋白互作界面，为理解其生物学功能及药物设计提供了结构基础。
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注：实际研究中，SSSCA1(Sperm-Specific Antigen 1)可能与生殖系统或某些癌症相关，但具体文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of SSSCA1 Recombinant Protein**
SSSCA1 (Scleroderma Systematic Sclerosis Cancer Antigen 1), also known as CENP-X, is a protein initially identified as an autoantigen in patients with systemic sclerosis (scleroderma), a rare autoimmune disorder characterized by fibrosis and vascular abnormalities. It later gained attention for its dual association with cancer biology and chromosomal regulation. Structurally, SSSCA1 is a component of the centromere protein complex, specifically interacting with CENP-T and CENP-W to form a critical part of the constitutive centromere-associated network (CCAN), which ensures proper kinetochore assembly and chromosome segregation during mitosis.
The recombinant SSSCA1 protein is produced using genetic engineering techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its recombinant form retains functional epitopes, making it valuable for studying autoimmune responses, centromere dynamics, and mitotic regulation. In research, SSSCA1 has been implicated in cancer progression due to its overexpression in certain malignancies, potentially contributing to chromosomal instability and tumorigenesis. Additionally, it serves as a biomarker in autoimmune diagnostics, aiding in the detection of autoantibodies in scleroderma patients.
Studies using recombinant SSSCA1 have advanced understanding of its roles in cell division, DNA repair, and immune dysregulation, highlighting its therapeutic potential as a target for cancer or autoimmune therapies. Its recombinant availability continues to support antibody development, mechanistic studies, and diagnostic assay optimization.
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