首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人生长抑制蛋白4(ING4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ING4含量。ING4（生长抑制因子4）是一种肿瘤抑制因子，其基因表达下调与多种肿瘤的发生发展密切相关。ING4通过抑制肿瘤细胞生长、诱导细胞凋亡和调节肿瘤细胞周期等途径发挥抑癌作用。研究显示，ING4基因在肺癌、胃癌等肿瘤中表达下调，且其表达水平与肿瘤的恶性程度和预后相关。ING4的抑癌机制可能与诱导肿瘤细胞凋亡、抑制肿瘤血管生成和调节细胞周期有关。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于科研领域中对ING4表达水平的定量分析，例如在肿瘤生物学研究中探索其与细胞增殖、转移的分子机制，或在疾病模型中评估ING4动态变化与病理进程的关联性，为基因功能研究及信号通路分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Brain my036 protein ELISA Kit; Candidate tumor suppressor p33 ING 1 homolog ELISA Kit; Candidate tumor suppressor p33 ING1 homolog ELISA Kit; D6Wsu147e ELISA Kit; D6Xrf92 ELISA Kit; ING 1 like protein ELISA Kit; ING 4 ELISA Kit; ING1 like protein ELISA Kit; ING4 ELISA Kit; ING4_HUMAN ELISA Kit; Inhibitor of growth family member 4 ELISA Kit; Inhibitor of growth family member 4 long isoform ELISA Kit; Inhibitor of growth protein 4 ELISA Kit; MGC12557 ELISA Kit; my036 ELISA Kit; p29 ING 4 ELISA Kit; p29 ING4 ELISA Kit; p29ING4 ELISA Kit
缩写：ING4
Uniprot No.：Q9UNL4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ING4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of human ING4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-93 | ||||
| EDTA plasma (n=4) | 104 | 99-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.7142.6422.6782.56515001.5381.5441.5411.4287500.7490.7880.7690.6563750.4910.5000.4960.383187.50.3010.3120.3070.194940.2240.2320.2280.115470.1830.1740.1790.06600.1140.1120.113 | ||||||
货期：3-5 working days
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