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产品描述:犬抑制素β-B(Inhbb)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的Inhbb含量。犬抑制素β-B(Inhbb)是一种在多种恶性肿瘤中发挥重要作用的蛋白,其上调与胃癌患者预后不良相关。研究发现,Inhbb通过调节成纤维细胞的功能,影响肿瘤微环境,进而促进胃癌细胞增殖、迁移和侵袭。试剂盒检测范围为5 pg/mL-1000 pg/mL,该产品广泛应用于生殖生物学、内分泌学研究领域,特别适用于卵巢功能评估、睾丸发育调控、妊娠相关激素变化等科研方向,亦可作为生殖系统疾病动物模型的生物标志物检测工具,为犬类繁殖力研究及生殖健康机制探索提供可靠的技术支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:Inhbb
Uniprot No.:F1PB74
种属:Canis lupus familiaris (Dog) (Canis familiaris)
样本类型:serum, plasma, tissue homogenates
检测范围:5 pg/mL-1000 pg/mL
灵敏度:2.5 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of canine Inhbb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 99 | ||||
Range % | 93-104 | |||||
1:2 | Average % | 90 | ||||
Range % | 85-94 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-96 | |||||
1:8 | Average % | 100 | ||||
Range % | 96-104 |
回收率:
The recovery of canine Inhbb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-99 | ||||
EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2Average10000.1680.1630.1662500.3110.3210.316800.5090.5180.514200.7980.8240.81151.3061.3191.31302.0141.9862.000 |
货期:3-5 working days
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