首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰岛素样因子3(INSL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的INSL3含量。INSL3是一种胰岛素样激素，主要在性腺组织中表达，与睾丸沉降、生殖细胞存活、骨代谢调控相关。在绵羊中，INSL3基因可能与角的有无有关，研究发现其表达与公羊睾丸和母羊卵巢组织的差异显著，且存在多个潜在功能位点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可广泛应用于生殖生物学领域研究，包括睾丸发育机制探索、内分泌调控网络解析、生殖相关疾病动物模型评估，以及药物或环境因素对性腺功能影响的体外实验分析。科研人员可通过检测不同样本中 INSL3 的动态变化，深入探究其在雄性生殖健康中的作用机制，为相关基础研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Insl3 ELISA Kit; Rlf ELISA Kit; Insulin-like 3 ELISA Kit; Leydig insulin-like peptide ELISA Kit; Ley-I-L ELISA Kit; Relaxin-like factor) [Cleaved into: Insulin-like 3 B chain; Insulin-like 3 A chain] ELISA Kit
缩写：INSL3
Uniprot No.：Q9WUK0
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat INSL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 94-99 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 83-96 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of rat INSL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 99-105 | |||||
| EDTA plasma (n=4) | 92 | 85-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected101.9781.9441.9611.75951.4361.5451.4911.2892.51.0211.0061.0140.8121.250.6650.6450.6550.4530.6250.4230.4120.4180.2160.3120.3120.3250.3190.1170.1560.2550.2700.2630.06100.1980.2050.202 | |||||||
货期：3-5 working days
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