首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒,为竞争法ELISA试剂盒，定量检测血清、血浆、tissue 匀浆、组织培养上清液、细胞裂解物样本中的IP3含量。IP3（肌醇三磷酸）是一种重要的细胞信号分子，由磷脂酶C（PLC）水解磷脂酰肌醇-4,5-二磷酸（PIP2）产生。它作为第二信使，通过结合内质网上的IP3受体，触发钙离子释放，从而调节细胞内钙信号，影响多种细胞反应，如平滑肌收缩、血小板聚集等。研究IP3信号转导机制有助于理解相关疾病的发生和发展，为疾病治疗提供新靶点。试剂盒检测范围为5 pg/mL-1000 pg/mL，该产品适用于神经生物学、心血管疾病模型、药物作用机制等研究领域，可用于探索细胞内信号通路、评估化合物干预效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IP3
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell culture supernates, cell lysates
检测范围：5 pg/mL-1000 pg/mL
灵敏度：2.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-104 | |||||
回收率：
| The recovery of rat IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-105 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.1410.1430.142 2500.2700.2610.266 800.4230.4400.432 200.7440.7590.752 51.1561.1621.159 02.1992.2142.207 | ||||||
货期：3-5 working days
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