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产品描述：牛泛素样蛋白ISG15(ISG15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ISG15含量。ISG15 是一个重要靶点。它是一种泛素样蛋白，在机体免疫应答等方面有重要作用。其研究机制主要围绕它与蛋白质的结合修饰，参与调控免疫信号通路、抗病毒反应等，深入探究 ISG15 有助于揭示疾病发生发展机理并为相关治疗提供思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于研究牛类病毒感染机制（如口蹄疫、牛病毒性腹泻等）、免疫应激反应评估、疫苗开发中的免疫效果监测，以及炎症相关疾病模型的分子机制探索等科研领域，为动物免疫学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ISG15 ELISA Kit; G1P2 ELISA Kit; ISG17 ELISA Kit; UCRP ELISA Kit; Ubiquitin-like protein ISG15 ELISA Kit; Interferon-stimulated gene product 17 ELISA Kit; Ubiquitin cross-reactive protein ELISA Kit; BoUCRP ELISA Kit
缩写：ISG15
Uniprot No.：O02741
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 85-102 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 83-99 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 83-103 | ||||||
回收率：
| The recovery of bovine ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 93 | 86-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.1051.9952.0501.871101.6541.6271.6411.46251.1531.1851.1690.9902.50.7720.7570.7650.5861.250.5370.5340.5360.3570.6250.3640.3550.3600.1810.3120.2620.2750.2690.09000.1770.1810.179 | |||||||
货期：3-5 working days
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