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产品描述:人整合素α2(ITGA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ITGA2含量。ITGA2 是一种重要的靶点。它在细胞黏附、迁移等过程中起关键作用。其研究机制主要聚焦于与配体结合后触发的信号传导通路,影响细胞行为。深入研究 ITGA2,可为肿瘤、心血管疾病等的治疗提供新的思路与靶点选择。试剂盒检测范围为0.235 ng/mL-15 ng/mL,可广泛应用于科研领域,例如探究ITGA2在肿瘤微环境中的促侵袭机制、炎症性疾病中的细胞迁移调控,或作为组织再生研究中的分子标记物检测工具,为体外实验研究提供可靠的数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:BR ELISA Kit; CD 49b ELISA Kit; CD49 antigen like family member B ELISA Kit; CD49 antigen-like family member B ELISA Kit; CD49b ELISA Kit; CD49b antigen ELISA Kit; Collagen receptor ELISA Kit; DX5 ELISA Kit; Glycoprotein Ia deficiency included ELISA Kit; GP Ia ELISA Kit; GP Ia deficiency; included ELISA Kit; GPIa ELISA Kit; HPA 5 included ELISA Kit; HPA5 included ELISA Kit; Human platelet alloantigen system 5 ELISA Kit; Integrin alpha 2 ELISA Kit; Integrin alpha II ELISA Kit; Integrin alpha-2 ELISA Kit; Integrin; alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) ELISA Kit; ITA2_HUMAN ELISA Kit; ITGA2 ELISA Kit; Platelet alloantigen Br(a); included ELISA Kit; Platelet antigen Br ELISA Kit; Platelet glycoprotein GPIa ELISA Kit; Platelet glycoprotein Ia ELISA Kit; Platelet glycoprotein Ia/IIa ELISA Kit; Platelet membrane glycoprotein Ia ELISA Kit; Platelet receptor for collagen; deficiency of; included ELISA Kit; Very late activation protein 2 receptor alpha 2 subunit ELISA Kit; VLA 2 alpha chain ELISA Kit; VLA 2 ELISA Kit; VLA 2 subunit alpha ELISA Kit; VLA-2 subunit alpha ELISA Kit; VLA2 ELISA Kit; VLA2 receptor alpha 2 subunit ELISA Kit; VLAA2 ELISA Kit
缩写:ITGA2
Uniprot No.:P17301
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.235 ng/mL-15 ng/mL
灵敏度:0.058 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:5 | Average % | 92 | |||||
Range % | 88-96 | ||||||
1:10 | Average % | 104 | |||||
Range % | 100-107 | ||||||
1:20 | Average % | 92 | |||||
Range % | 89-96 | ||||||
1:40 | Average % | 95 | |||||
Range % | 93-97 |
回收率:
The recovery of human ITGA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 98 | 93-104 | |||||
EDTA plasma (n=4) | 93 | 90-96 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected152.4922.5442.5182.3397.52.1502.2312.1912.0123.751.5671.6251.5961.4171.880.9440.9550.9500.7710.940.5480.5870.5680.3890.470.3530.3520.3530.1740.2350.2540.2660.2600.08100.1770.1800.179 |
货期:3-5 working days
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