首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠整合素αM(Itgam/CD11b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Itgam/CD11b含量。Itgam/CD11b是重要的靶点。其背景在于在免疫细胞表面表达，参与免疫应答等生理过程。研究机制主要聚焦于其在炎症、免疫相关疾病中的作用，如调节白细胞黏附、迁移等，有望为相关疾病治疗方案的开发提供新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，通过包被特异性捕获抗体和标记检测抗体的协同作用，实现对目标蛋白的高特异性识别，可广泛应用于科研领域中小鼠免疫细胞功能研究、炎症性疾病模型的分子机制探索、药物干预实验的效应评估等场景，特别适用于巨噬细胞、中性粒细胞等髓系细胞在感染、自身免疫病或肿瘤微环境中的Itgam/CD11b表达水平分析，为免疫调控机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Itgam ELISA Kit; Integrin alpha-M ELISA Kit; CD11 antigen-like family member B ELISA Kit; CR-3 alpha chain ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; CD antigen CD11b ELISA Kit
缩写：Itgam/CD11b
Uniprot No.：P05555
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Itgam/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 86 | |||||
| Range % | 81-90 | ||||||
| 1:200 | Average % | 103 | |||||
| Range % | 99-108 | ||||||
| 1:400 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
回收率：
| The recovery of mouse Itgam/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.6032.5272.5652.3765001.9201.8651.8931.7042501.3671.4411.4041.2151250.9050.9280.9170.72862.50.5860.5740.5800.39131.20.4390.4190.4290.24015.60.2910.3040.2980.10900.1860.1920.189 | |||||||
货期：3-5 working days
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