首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人整合素β3(ITGB3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ITGB3含量。ITGB3 是一种重要的靶点。它参与细胞黏附、迁移等过程。在研究机制上，其与多种信号通路交互，在肿瘤转移、血栓形成等病理过程中发挥关键作用。对它的深入研究有助于开发针对相关疾病，如癌症、心血管疾病的新型治疗方法。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外定量检测多种生物样本中的ITGB3表达水平；可满足心血管疾病、肿瘤微环境研究、炎症反应机制等基础科研领域的需求，为探索ITGB3在病理生理过程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ITGB3; GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61
缩写：ITGB3
Uniprot No.：P05106
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-108 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human ITGB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 97 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.4332.5792.5062.36240001.8871.9361.9121.76820001.4061.4881.4471.30310000.9491.0150.9820.8385000.6350.6690.6520.5082500.3710.3450.3580.2141250.2620.2470.2550.11100.1400.1480.144 | ||||||
货期：3-5 working days
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