首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFR-2/Flk-1含量。VEGFR-2/Flk-1是重要的血管内皮生长因子受体。其背景在于在血管生成过程里作用关键。研究机制为，它能与配体结合，激活下游信号通路，促进内皮细胞的增殖、迁移和存活，是肿瘤血管生成相关研究及药物开发的热门靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，支持科研人员开展血管生成机制、抗血管药物筛选及病理模型评估等实验，适用于肿瘤微环境研究、心血管疾病动物模型的组织表达分析以及炎症相关血管病变的定量检测。组织匀浆检测功能特别适配器官特异性研究需求，为体外实验提供高灵敏度的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kdr ELISA Kit; Flk1 ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR-2 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; CD antigen CD309 ELISA Kit
缩写：KDR
Uniprot No.：O08775
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-105 | |||||
回收率：
| The recovery of rat VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 107 | 101-113 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.9542.9112.9332.783501.9331.9731.9531.803251.3651.4801.4231.27312.50.8940.8850.8900.7406.250.5580.5990.5790.4293.120.3130.3280.3210.1711.560.2220.2300.2260.07600.1450.1540.150 | ||||||
货期：3-5 working days
×
