首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肿瘤转移抑制基因KISS1蛋白（KISS1/Kisspeptin 1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KISS1含量。KISS1是一种肿瘤转移抑制基因，位于人染色体1q32-q41，由4个外显子组成。它通过抑制细胞趋化性、增强成簇黏附激酶的表达和活性来抑制肿瘤转移。KISS1基因在多种生命过程中起作用，如性发育、肿瘤转移和血糖调节。其研究机制涉及转录调控因子和表观遗传因素。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域中对大鼠肿瘤模型、细胞实验或生物样本中KISS1蛋白表达水平的定量分析，可用于探索肿瘤转移机制、评估干预药物对KISS1通路的影响，或结合其他分子标志物进行肿瘤微环境研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kiss1 ELISA Kit; Metastasis-suppressor KiSS-1 ELISA Kit; Kisspeptin-1) [Cleaved into: Metastin; Kisspeptin-10 ELISA Kit; Metastin45-54)] ELISA Kit
缩写：KISS1
Uniprot No.：Q7TSB7
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 82-88 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 87-96 | ||||||
| 1:4 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
回收率：
| The recovery of rat KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 84-90 | |||||
| EDTA plasma (n=4) | 92 | 90-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.6312.7542.6932.531 51.9781.9621.9701.808 2.51.2861.2681.2771.115 1.250.7260.7350.7310.569 0.6250.5080.5120.5100.348 0.3120.3860.3960.3910.229 0.1560.2320.2450.2390.077 00.1610.1630.162 | |||||||
货期：3-5 working days
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