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产品描述：人激肽释放酶10(KLK 10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的KLK10含量。试剂盒检测范围为1.675 ng/mL-40 ng/mL，操作简便、特异性强，可广泛用于细胞生物学、分子机制研究及疾病模型构建等科研场景，例如通过检测血清或血浆中KLK 10浓度变化，为肿瘤微环境、炎症反应或代谢调控等基础研究提供数据支持，所有应用均限于科学研究目的。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Breast normal epithelial cell associated serine protease ELISA Kit; Kallikrein related peptidase 10 ELISA Kit; Kallikrein-10 ELISA Kit; Kallikrein10 ELISA Kit; KLK 10 ELISA Kit; KLK10 ELISA Kit; KLK10_HUMAN ELISA Kit; NES 1 ELISA Kit; NES1 ELISA Kit; Normal epithelial cell specific 1 ELISA Kit; Normal epithelial cell-specific 1 ELISA Kit; Protease serine like 1 ELISA Kit; Protease serine-like 1 ELISA Kit; PRSS L1 ELISA Kit; PRSSL 1 ELISA Kit; PRSSL1 ELISA Kit
缩写：KLK10
Uniprot No.：O43240
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.675 ng/mL-40 ng/mL
灵敏度：0.7 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 85-106 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 88-97 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-103 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-101 | ||||||
回收率：
| The recovery of human KLK 10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 89-100 | |||||
| EDTA plasma (n=4) | 95 | 90-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected401.9562.0141.9851.931201.3371.3241.3311.277100.7960.8080.8020.7483.30.3420.3550.3490.2951.670.2110.2150.2130.15900.0530.0550.054 | |||||||
货期：3-5 working days
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