首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人II型角蛋白,细胞支架1(KRT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KRT1含量。KRT1 即角蛋白 1，是上皮细胞内中间丝网络的重要组成部分。其表达异常与多种皮肤疾病相关，如鱼鳞病等。研究机制上，主要围绕其在维持细胞结构、信号传导中的作用，通过影响细胞黏附、迁移等过程，来探究其在疾病发生发展中的影响。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于皮肤生物学研究、上皮细胞分化模型构建、疾病相关分子机制探索等科研场景，为研究KRT1在组织修复、肿瘤微环境调控等领域的功能提供了可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：67 kDa cytokeratin ELISA Kit; CK-1 ELISA Kit; CK1 ELISA Kit; Cytokeratin-1 ELISA Kit; Cytokeratin1 ELISA Kit; EHK ELISA Kit; EHK1 ELISA Kit; Epidermolytic hyperkeratosis 1 ELISA Kit; EPPK ELISA Kit; Hair alpha protein ELISA Kit; K1 ELISA Kit; K2C1_HUMAN ELISA Kit; Keratin ELISA Kit; Keratin type II cytoskeletal 1 ELISA Kit; Keratin-1 ELISA Kit; Keratin1 ELISA Kit; KRT 1 ELISA Kit; Krt1 ELISA Kit; KRT1A ELISA Kit; NEPPK ELISA Kit; type II cytoskeletal 1 ELISA Kit; Type II keratin Kb1 ELISA Kit; Type-II keratin Kb1 ELISA Kit
缩写：KRT1
Uniprot No.：P04264
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-93 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human KRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-91 | ||||
| EDTA plasma (n=4) | 92 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.8622.8982.8802.727 51.9911.9991.9951.842 2.51.1721.1981.1851.032 1.250.6400.6740.6570.504 0.6250.4250.4360.4310.278 0.3120.3530.3680.3610.208 0.1560.2600.2680.2640.111 00.1520.1540.153 | ||||||
货期：3-5 working days
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