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Human Kynurenine (KYN)ELISA Kit

  • 中文名： 人犬尿氨酸（KYN)ELISA Kit
货号: IPD-E13659h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人犬尿氨酸（KYN)酶联免疫试剂盒,竞争法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的KYN含量。试剂盒检测范围为7.8 pmol/ml-500 pmol/ml，产品适用于科研场景中KYN代谢机制研究、疾病模型评估及药物开发过程中的生物标志物检测，例如探索神经系统疾病动物模型的代谢变化或免疫调节药物对色氨酸代谢通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    KYN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine, tissue homogenates
  • 检测范围：
    7.8 pmol/ml-500 pmol/ml.
  • 灵敏度：
    3.9 pmol/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human KYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    90-99


    1:2

    Average %

    87


    Range %

    84-90


    1:4

    Average %

    92


    Range %

    88-96


    1:8

    Average %

    97


    Range %

    95-99


  • 回收率：

    The recovery of human KYN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    90-98


    EDTA plasma (n=4)

    92

    87-96


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pmol/ml

    OD1

    OD2

    Average



    500

    0.200

    0.202

    0.201



    250

    0.318

    0.338

    0.328



    125

    0.498

    0.470

    0.484



    62.5

    0.685

    0.664

    0.675



    31.2

    1.036

    1.016

    1.026



    15.6

    1.468

    1.404

    1.436



    7.8

    1.533

    1.657

    1.595



    0

    1.710

    1.723

    1.717




  • 货期：
    3-5 working days

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