首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人层粘连蛋白亚基α-4(LAMA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LAMA4含量。LAMA4是一个重要靶点。它是层粘连蛋白α4亚基，在细胞黏附、迁移和组织发育等过程有重要作用。研究机制方面，主要聚焦其与细胞表面受体相互作用调节细胞行为，在肿瘤、心血管疾病等病理机制研究中有潜在意义。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于多种科研场景，包括探究心血管疾病、肿瘤转移或纤维化疾病中细胞外基质的动态变化，分析组织再生过程中LAMA4的表达调控机制，以及评估实验模型中基质重塑相关分子通路的功能。为体外研究LAMA4在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CMD1JJ ELISA Kit; DKFZp686D23145 ELISA Kit; LAMA3 ELISA Kit; LAMA3, formerly ELISA Kit; LAMA4 1 ELISA Kit; LAMA4 ELISA Kit; LAMA4*-1 ELISA Kit; LAMA4_HUMAN ELISA Kit; Laminin alpha 4 ELISA Kit; Laminin alpha 4 chain ELISA Kit; Laminin subunit alpha 4 precursor ELISA Kit; Laminin subunit alpha-4 ELISA Kit; Laminin-14 subunit alpha ELISA Kit; Laminin-8 subunit alpha ELISA Kit; Laminin-9 subunit alpha ELISA Kit
缩写：LAMA4
Uniprot No.：Q16363
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LAMA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 88-99 | ||||||
| 1:40 | Average % | 104 | |||||
| Range % | 98-108 | ||||||
| 1:80 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:160 | Average % | 99 | |||||
| Range % | 90-103 | ||||||
回收率：
| The recovery of human LAMA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 84-97 | |||||
| EDTA plasma (n=4) | 101 | 93-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.6732.5862.6302.5357502.1802.1452.1632.0683751.7861.7121.7491.654187.51.2011.2391.2201.125940.6530.6480.6510.556470.4150.4220.4190.32423.50.2520.2630.2580.16300.0930.0970.095 | |||||||
货期：3-5 working days
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