首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人溶酶体相关膜蛋白2(HLAMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LAMP2 含量。LAMP2是一种溶酶体相关膜蛋白，对溶酶体结构和功能至关重要。其基因突变与达农病相关，该病以自噬空泡积聚和器官功能障碍为特征。LAMP2通过介导自噬体与溶酶体的融合，参与细胞内物质的降解和细胞稳态的维持。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础医学研究中HLAMP - 2的生理功能探索、疾病模型构建（如代谢紊乱或神经退行性疾病）以及药物干预效果评估等场景，助力溶酶体相关分子机制及病理生理学的研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LAMP2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96; CD antigen CD107b
缩写：HLAMP-2
Uniprot No.：P13473
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HLAMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 96 | |||||
| Range % | 91-100 | ||||||
回收率：
| The recovery of human HLAMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 82-91 | |||||
| EDTA plasma (n=4) | 93 | 89-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.4822.5262.5042.320101.8701.9121.8911.70751.1451.1691.1570.9732.50.7150.6930.7040.5201.250.4100.4400.4250.2410.6250.2900.2710.2810.0970.3120.2240.2370.2310.04700.1800.1880.184 | |||||||
货期：3-5 working days
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