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Human Plastin-2(LCP1) ELISA kit

  • 中文名: 人网素-2(LCP1)酶联免疫试剂盒
货号: IPD-EL012824HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人网素-2(LCP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的LCP1含量。人网素-2(LCP1)是一种肌动蛋白结合调节因子,在T细胞激活中发挥关键作用,通过TCR/CD3和CD2或CD28响应共刺激信号,调节IL2RA/CD25和CD69的细胞表面表达,并参与免疫反应和T细胞激活。其研究机制涉及与AIF1和肌动蛋白的相互作用,以及调节细胞表面信号分子的表达。试剂盒检测范围为15.6 pg/ml-1000 pg/ml,该产品适用于基础科研领域,如探究LCP1在免疫细胞功能、肿瘤微环境调控或炎症性疾病模型中的表达特征,亦可结合其他分子标记物进行多维度机制研究,为体外实验及动物模型样本分析提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    CP64 ELISA Kit; L plastin ELISA Kit; L-plastin ELISA Kit; Larval cuticle protein 1 ELISA Kit; LC64P ELISA Kit; LCP-1 ELISA Kit; LCP1 ELISA Kit; LPL ELISA Kit; Lplastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; Plastin 2 ELISA Kit; Plastin-2 ELISA Kit; PLS2 ELISA Kit; PLSL_HUMAN ELISA Kit
  • 缩写:
    LCP1
  • Uniprot No.:
    P13796
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    15.6 pg/ml-1000 pg/ml.
  • 灵敏度:
    3.9 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    106


    Range %

    102-112


    1:2

    Average %

    96


    Range %

    90-102


    1:4

    Average %

    98


    Range %

    91-105


    1:8

    Average %

    91


    Range %

    87-96


  • 回收率:

    The recovery of human LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    88-97


    EDTA plasma (n=4)

    99

    92-106


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    1.998

    1.874

    1.936

    1.813


    500

    1.264

    1.257

    1.260

    1.137


    250

    0.841

    0.828

    0.834

    0.711


    125

    0.518

    0.529

    0.523

    0.400


    62.5

    0.383

    0.391

    0.387

    0.264


    31.2

    0.295

    0.283

    0.289

    0.166


    15.6

    0.199

    0.189

    0.194

    0.071


    0

    0.122

    0.125

    0.123




  • 货期:
    3-5 working days

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