首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠L-乳酸脱氢酶(L-LDH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDHA含量。LDHA即乳酸脱氢酶A，在糖酵解途径中发挥关键作用，与细胞能量代谢密切相关。异常表达的LDHA与肿瘤的发生、发展、转移及预后不良相关。目前研究聚焦于抑制LDHA活性或表达来调控肿瘤糖代谢，有望为肿瘤治疗提供新策略。试剂盒检测范围为39 mU/mL-2500 mU/mL，该产品可应用于科研场景中药物毒性评估、疾病模型构建、代谢机制研究等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
缩写：LDHA
Uniprot No.：P04642
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：39 mU/mL-2500 mU/mL
灵敏度：9.77 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-100 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected25002.7542.9522.8532.67912502.2182.3762.2972.1236251.5341.5841.5591.385312.51.0221.0391.0310.8571560.5870.6240.6060.432780.4120.4300.4210.247390.2860.2790.2830.10900.1730.1750.174 | ||||||
货期：3-5 working days
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