首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人L乳酸脱氢酶B链(LDHB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）、尿液.样本中的LDHB含量。LDHB即乳酸脱氢酶B，是乳酸脱氢酶的亚基之一。它参与糖酵解和乳酸代谢过程。研究发现其在多种肿瘤中表达异常，可能通过调节细胞代谢微环境、影响肿瘤细胞增殖凋亡等机制，在肿瘤发生发展中发挥作用，是潜在的肿瘤治疗靶点。试剂盒检测范围为23.5 mU/mL-1500 mU/mL，该产品适用于体外科研实验中多种生物样本的LDHB活性检测，可服务于细胞代谢研究、疾病模型能量通路分析、肿瘤微环境评估等基础研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Epididymis secretory protein Li 281 ELISA Kit; HEL S 281 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase B chain ELISA Kit; Lactate Dehydrogenase B ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH-B ELISA Kit; LDH-H ELISA Kit; LDHB ELISA Kit; LDHB_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHH ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-46 ELISA Kit; TRG-5 ELISA Kit; TRG5 ELISA Kit
缩写：LDHB
Uniprot No.：P07195
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF), urine.
检测范围：23.5 mU/mL-1500 mU/mL
灵敏度：5.8 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LDHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 97-103 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-90 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of human LDHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 100-106 | |||||
| EDTA plasma (n=4) | 89 | 85-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected 15002.1842.2032.1942.038 7501.7061.6971.7021.546 3751.2151.2411.2281.072 187.50.7570.7380.7480.592 940.4420.4680.4550.299 470.3190.2980.3090.153 23.50.2440.2310.2380.082 00.1590.1530.156 | |||||||
货期：3-5 working days
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