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产品描述：人D-乳酸脱氢酶(D-LDH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDHD含量。人D-乳酸脱氢酶（D-LDH）在肿瘤代谢中起关键作用，其研究机制涉及在厌氧条件下催化D-乳酸生成丙酮酸，影响肿瘤生长和代谢。D-LDH基因敲除可提高D-乳酸产量，纯度高达99%以上，为D-乳酸工业化生产提供新途径。试剂盒检测范围为31.25 mIU/mL-2000 mIU/mL，该产品可广泛应用于肠道功能研究、代谢通路探索、疾病模型构建等方向，例如评估缺血再灌注损伤、肠道屏障功能障碍或微生物代谢研究中D - LDH的动态变化，为能量代谢调控机制及病理生理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
缩写：LDHD
Uniprot No.：Q86WU2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 mIU/mL-2000 mIU/mL
灵敏度：7.8 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 87-103 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-103 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 86-105 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 84-100 | |||||
回收率：
| The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-106 | ||||
| EDTA plasma (n=4) | 96 | 86-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected20002.4182.3852.4022.26510001.7731.7281.7511.6145000.9361.0020.9690.8322500.6240.6510.6380.5011250.4070.4220.4150.27862.50.2820.2870.2850.14831.250.2530.2640.2590.12200.1350.1390.137 | ||||||
货期：3-5 working days
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