首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠低密度脂蛋白(LDL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDL含量。LDL即低密度脂蛋白，是一种运载胆固醇进入外周组织细胞的脂蛋白颗粒。其水平升高易致胆固醇沉积于动脉壁，引发动脉粥样硬化等心血管疾病。研究聚焦于降低LDL水平机制，像抑制其合成、促进代谢清除等，以防治相关病症。试剂盒检测范围为7.8 ng/mL-500 ng/mL，该产品可广泛应用于小鼠模型的血脂代谢研究、动脉粥样硬化病理机制探索以及降脂药物或功能性食品的体外药效评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：LDL
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 98-104 | |||||
回收率：
| The recovery of mouse LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 98 | 93-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 5000.1570.1590.158 2500.2190.2390.229 1250.3340.3150.325 62.50.5370.5230.530 31.20.8750.8980.887 15.61.1991.1871.193 7.81.5581.5781.568 02.5032.5342.519 | ||||||
货期：3-5 working days
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