首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人白细胞衍生趋化因子-2(LECT2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、组织培养上清液样本中的LECT2含量。LECT2是一种白细胞来源的趋化因子,与肝纤维化密切相关。其研究机制表明,LECT2在正常生理条件下主要表达于肝脏细胞和中性粒细胞中,在炎症条件下可加重肝纤维化。研究还发现,LECT2通过调控巨噬细胞增强对细菌的清除能力,并可能作为肝纤维化的诊断和治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL,该产品可广泛用于基础医学研究中LECT2表达动态监测,例如肝脏疾病模型中的蛋白分泌研究、炎症微环境分析、细胞因子网络互作机制探索,以及药物干预实验中的生物标志物定量评估,为免疫调控机制和病理生理学研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:chm II ELISA Kit; chm2 ELISA Kit; Chondromodulin II ELISA Kit; hLECT2 ELISA Kit; LECT 2 ELISA Kit; LECT-2 ELISA Kit; LECT2 ELISA Kit; LECT2_HUMAN ELISA Kit; Leukocyte cell derived chemotaxin 2 ELISA Kit; Leukocyte cell-derived chemotaxin-2 ELISA Kit; MGC126628 ELISA Kit
缩写:LECT2
Uniprot No.:O14960
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell culture supernates
检测范围:1.56 ng/mL-100 ng/mL
灵敏度:0.39 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human LECT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 89-97 | |||||
1:2 | Average % | 98 | ||||
Range % | 95-102 | |||||
1:4 | Average % | 102 | ||||
Range % | 94-105 | |||||
1:8 | Average % | 95 | ||||
Range % | 90-99 |
回收率:
The recovery of human LECT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 88-97 | ||||
EDTA plasma (n=4) | 99 | 95-105 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected1002.9023.0993.0012.809502.2472.3922.3202.128251.5631.4921.5281.33612.50.7840.7710.7780.5866.250.4230.4550.4390.2473.120.3450.3400.3430.1511.560.2330.2530.2430.05100.1860.1970.192 |
货期:3-5 working days
×