| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYT13 |
| Uniprot No | Q7L8C5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-426aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSCRHMHPK KGLLPRDQDP DLEKAKPSLL GSAQQFNVKK STEPVQPRAL LKFPDIYGPR PAVTAPEVIN YADYSLRSTE EPTAPASPQP PNDSRLKRQV TEELFILPQN GVVEDVCVME TWNPEKAASW NQAPKLHYCL DYDCQKAELF VTRLEAVTSN HDGGCDCYVQ GSVANRTGSV EAQTALKKRQ LHTTWEEGLV LPLAEEELPT ATLTLTLRTC DRFSRHSVAG ELRLGLDGTS VPLGAAQWGE LKTSAKEPSA GAGEVLLSIS YLPAANRLLV VLIKAKNLHS NQSKELLGKD VSVKVTLKHQ ARKLKKKQTK RAKHKINPVW NEMIMFELPD DLLQASSVEL EVLGQDDSGQ SCALGHCSLG LHTSGSERSH WEEMLKNPRR QIAMWHQLHL |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYT13(突触结合蛋白13)重组蛋白的模拟参考文献示例(基于公开研究领域常见主题的合理推测)：
1. **文献名称**：*"Recombinant SYT13 Protein Expression and Its Role in Calcium-Dependent Membrane Binding"*
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组SYT13蛋白，通过体外实验证实其C2结构域具有钙离子依赖性脂质结合能力，提示SYT13可能在细胞膜融合过程中发挥调控作用。
2. **文献名称**：*"Structural Characterization of the SYT13 C2 Domain by X-ray Crystallography"*
**作者**：Wang Y, et al.
**摘要**：利用X射线晶体学解析了SYT13蛋白C2结构域的三维结构，揭示了其独特的钙离子结合位点，为理解其与磷脂膜的相互作用机制提供了结构基础。
3. **文献名称**：*"SYT13 Recombinant Protein Promotes Cancer Cell Invasion via EGFR Signaling Pathway"*
**作者**：Kim H, et al.
**摘要**：通过体外细胞实验发现，外源性添加重组SYT13蛋白可增强乳腺癌细胞的迁移和侵袭能力，进一步研究表明该作用可能通过激活EGFR信号通路实现。
4. **文献名称**：*"Development of a SYT13-Specific Monoclonal Antibody Using Recombinant Protein Immunization"*
**作者**：Sato R, et al.
**摘要**：利用纯化的重组SYT13蛋白免疫小鼠，成功制备了高特异性的单克隆抗体，该抗体可用于SYT13在组织样本中的定位及表达水平检测。
**注**：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。
**Background of SYT13 Recombinant Protein**
Synaptotagmin-13 (SYT13) is a member of the synaptotagmin family, a group of membrane-trafficking proteins characterized by calcium-binding C2 domains. While synaptotagmins are best known for their roles in synaptic vesicle exocytosis and calcium-triggered neurotransmitter release, SYT13 represents a less-studied isoform with distinct functional and structural features. Unlike classical synaptotagmins (e.g., SYT1 or SYT7), SYT13 lacks a transmembrane domain and is instead anchored to membranes via lipid modifications or protein interactions. This unique property suggests divergent roles in intracellular signaling, membrane dynamics, or secretory pathways.
SYT13 is widely expressed in various tissues, including the brain, pancreas, and endocrine cells, implicating its potential involvement in neurodevelopment, hormone secretion, or cancer progression. Studies have linked SYT13 to cellular processes such as vesicle trafficking, cytoskeletal organization, and apoptosis regulation. Its C2 domains, though structurally similar to those in other synaptogmins, exhibit altered calcium-binding properties, possibly enabling specialized signaling functions.
Recombinant SYT13 protein is engineered for *in vitro* studies to dissect its biochemical interactions, calcium-dependent activity, and structural motifs. Produced via bacterial or mammalian expression systems, it is purified for applications like antibody development, binding assays, or functional studies. Research on SYT13 has gained momentum due to its potential associations with diseases, including neurodevelopmental disorders and cancers, where aberrant expression or mutations have been observed. However, its precise physiological and pathological mechanisms remain poorly understood, necessitating further exploration using recombinant tools. Overall, SYT13 represents an intriguing target for unraveling novel regulatory pathways in cell biology and disease.
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